Usefulness of protein analysis for detecting pathologies in bone remains.
Identifieur interne : 000013 ( Main/Exploration ); précédent : 000012; suivant : 000014Usefulness of protein analysis for detecting pathologies in bone remains.
Auteurs : Cristina Pérez-Martínez [Espagne] ; Gemma Prieto-Bonete [Espagne] ; María D. Pérez-Cárceles [Espagne] ; Aurelio Luna [Espagne]Source :
- Forensic science international [ 1872-6283 ] ; 2016.
English descriptors
- KwdEn :
- Adult, Aged, Aged, 80 and over, Anemia (diagnosis), Bacteria (isolation & purification), Biomarkers (analysis), Cadaver, Chromatography, High Pressure Liquid, Female, Femur (chemistry), Forensic Anthropology, Humans, Male, Mass Spectrometry, Middle Aged, Neoplasms (diagnosis), Proteins (analysis), Tibia (chemistry), Viruses (isolation & purification), Young Adult.
- MESH :
- chemical , analysis : Biomarkers, Proteins.
- chemistry : Femur, Tibia.
- diagnosis : Anemia, Neoplasms.
- isolation & purification : Bacteria, Viruses.
- Adult, Aged, Aged, 80 and over, Cadaver, Chromatography, High Pressure Liquid, Female, Forensic Anthropology, Humans, Male, Mass Spectrometry, Middle Aged, Young Adult.
Abstract
Forensic pathology often uses osteobiography, which involves biological profiles based on a determination of the age, sex, constitution, pathological states and other anomalies (paleopathology) of subjects for identification purposes. In this paper, proteins were analysed in bone remains. A total of 45 long bones from 45 different cadavers (29 males, 16 females) with a mean age of 66.31 years (S.D.=19.48, range 20-97) were used to search for pathological biomarkers which are closely related to several diseases. The bones were removed from the cement niches of a cemetery in Murcia (south-eastern Spain), where they had lain for between 18 and 45 years (mean time 25.84 years, S.D.=8.91). After a specific extraction using Tris-Urea buffer, were measured using HPLC/MS/MS. Our results show that proteins resulting from tumoral diseases and bacterial and viral pathogens can be detected and identified in the skeletal remains, making them useful pathological biomarkers for constructing biological profiles.
DOI: 10.1016/j.forsciint.2015.11.009
PubMed: 26674668
Affiliations:
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Le document en format XML
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Pérez-Cárceles</name><affiliation wicri:level="1"><nlm:affiliation>Department of Legal and Forensic Medicine, University of Murcia, Murcia, Spain. Electronic address: mdperez@um.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Legal and Forensic Medicine, University of Murcia, Murcia</wicri:regionArea><wicri:noRegion>Murcia</wicri:noRegion></affiliation></author><author><name sortKey="Luna, Aurelio" sort="Luna, Aurelio" uniqKey="Luna A" first="Aurelio" last="Luna">Aurelio Luna</name><affiliation wicri:level="1"><nlm:affiliation>Department of Legal and Forensic Medicine, University of Murcia, Murcia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Legal and Forensic Medicine, University of Murcia, Murcia</wicri:regionArea><wicri:noRegion>Murcia</wicri:noRegion></affiliation></author></analytic><series><title level="j">Forensic science international</title><idno type="eISSN">1872-6283</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Anemia (diagnosis)</term><term>Bacteria (isolation & purification)</term><term>Biomarkers (analysis)</term><term>Cadaver</term><term>Chromatography, High Pressure Liquid</term><term>Female</term><term>Femur (chemistry)</term><term>Forensic Anthropology</term><term>Humans</term><term>Male</term><term>Mass Spectrometry</term><term>Middle Aged</term><term>Neoplasms (diagnosis)</term><term>Proteins (analysis)</term><term>Tibia (chemistry)</term><term>Viruses (isolation & purification)</term><term>Young Adult</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Biomarkers</term><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Femur</term><term>Tibia</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Anemia</term><term>Neoplasms</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Cadaver</term><term>Chromatography, High Pressure Liquid</term><term>Female</term><term>Forensic Anthropology</term><term>Humans</term><term>Male</term><term>Mass Spectrometry</term><term>Middle Aged</term><term>Young Adult</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Forensic pathology often uses osteobiography, which involves biological profiles based on a determination of the age, sex, constitution, pathological states and other anomalies (paleopathology) of subjects for identification purposes. In this paper, proteins were analysed in bone remains. A total of 45 long bones from 45 different cadavers (29 males, 16 females) with a mean age of 66.31 years (S.D.=19.48, range 20-97) were used to search for pathological biomarkers which are closely related to several diseases. The bones were removed from the cement niches of a cemetery in Murcia (south-eastern Spain), where they had lain for between 18 and 45 years (mean time 25.84 years, S.D.=8.91). After a specific extraction using Tris-Urea buffer, were measured using HPLC/MS/MS. Our results show that proteins resulting from tumoral diseases and bacterial and viral pathogens can be detected and identified in the skeletal remains, making them useful pathological biomarkers for constructing biological profiles.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Perez Martinez, Cristina" sort="Perez Martinez, Cristina" uniqKey="Perez Martinez C" first="Cristina" last="Pérez-Martínez">Cristina Pérez-Martínez</name></noRegion><name sortKey="Luna, Aurelio" sort="Luna, Aurelio" uniqKey="Luna A" first="Aurelio" last="Luna">Aurelio Luna</name><name sortKey="Perez Carceles, Maria D" sort="Perez Carceles, Maria D" uniqKey="Perez Carceles M" first="María D" last="Pérez-Cárceles">María D. Pérez-Cárceles</name><name sortKey="Prieto Bonete, Gemma" sort="Prieto Bonete, Gemma" uniqKey="Prieto Bonete G" first="Gemma" last="Prieto-Bonete">Gemma Prieto-Bonete</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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